WebWell format Recommended loading volume Maximum loading volume Maximum protein load per band; 10-well WedgeWell: 40 μL: 60 μL: 0.5 µg: 12-well Wedgewell: 30 μL: 45 μL: 0.4 µg: 15-well Wedgewell: 20 μL: 35 μL: 0.25 µg: 17-well Wedgewell: 15 μL: 30 μL: 0.2 µg WebM01215L 12% 15 MOPS, MES Tris-Bicine, Tris-glycine 200―6 kDa M42015L 4-20% 15 MOPS, MES Tris-Bicine, Tris-glycine 250―3.5 kDa Table 2. Recommended loading volume and amount Maximum sample volume per well Recommended loading volume per well Total amount of protein per well Recommended amount of protein per well 10×10 …
Maximum Sample Volume Bio-Rad
WebFor Technical Service Call Your Local Bio-Rad Office or in the U.S. Call 1-800-4BIORAD (1-800-424-6723) Table of Contents ... Tris-HCl 12+2 18 well 26 well Prep+2 well IPG … WebFeb 20, 2024 · Simply adjust the mass of agarose in a given volume to make gels of other agarose concentrations (e.g., 2 g of agarose in 100 mL of TAE will make a 2% gel). ... Any bubbles can be pushed away from … philippe stinglhamber
SDS-PAGE - Assay-Protocol
WebChoose these Mini-PROTEAN 15-well combs for use with spacer plates with integrated 1.5 mm spacers for preparing gels with the Mini-PROTEAN Tetra Handcast Systems and … WebBio-Rad Products. Back. Bio-Rad Products Explore all. group1. Food Safety Testing; TSE Testing; Veterinary Diagnostics; Water Quality Testing; Wine Quality Testing; ... Mini-PROTEAN Comb, 15-well, 1.0 mm, 26 μl … WebMay 7, 2024 · So after determining the protein concentration via Bradford or BCA, you decide and determine the volume of the protein sample you want to use for Western. To … philippe stilite facebook