Web31 jul. 2014 · Leaderless mRNAs have been found to be rare in most bacteria – and previously only two leaderless mRNAs were identified in C. crescentus: dnaX and hemE. The presence of many leaderless mRNAs in C. crescentus and 171 in S. meliloti [33] suggests translation of leaderless mRNAs may occur more commonly in the alpha … Web17 mrt. 2024 · One quarter of mycobacterial mRNAs are leaderless, beginning with a 5′-AUG or GUG initiation codon. Leaderless mRNAs often encode unannotated short ORFs as the first gene of a polycistronic transcript. Here, we show that polycysteine-encoding leaderless short ORFs function as cysteine-responsive attenuators of operonic gene …
Selective Translation of Leaderless mRNAs by Specialized ... - Cell
WebThe recognition of leaderless mitochondrial mRNAs (7) by the 28S subunit during initiation does not rely on base pairing with rRNA (23). Consequently, the exit region of the mitoribosomal mRNA channel has very different features compared to bacteria, and the 12S rRNA lacks the anti-Shine-Dalgarno sequence. Webleaderless mRNA, indicating that the translational efficiency of this mRNA class can be modulated depending on the availability of components of the translational machinery. … courthouse evansville indiana
Primary transcriptome map of the hyperthermophilic archaeon ...
Web9 dec. 2024 · The system is capable of translating leaderless mRNAs encoding model proteins (DHFR and nanoLuciferase) or some mtDNA-encoded proteins. We show that a leaderless mRNA, encoding nanoLuciferase, is faithfully initiated without the need for any auxiliary factors other than IF-2mt and IF-3mt. Web12 jul. 2011 · Shine-Dalgarno (SD) signal has long been viewed as the dominant translation initiation signal in prokaryotes. Recently, leaderless genes, which lack 5'-untranslated regions (5'-UTR) on their mRNAs, have been shown abundant in archaea. However, current large-scale in silico analyses on initiation mechanisms in bacteria are mainly based on … Web16 aug. 2014 · A ribosome binding sequence (RBS) that conforms to the consensus GGDGRD is present in ~50% of the predicted mRNAs (Figure 1C). Initially, we identified 179 leaderless mRNAs based on having a 5′-UTR ≤8 nt long, most of which encode proteins with unknown functions although 15 have annotated functions related to RNA … courthouse exchange